Other

Part:BBa_J42040:Design

Designed by: Steve Selinsky   Group: iGEM06_Michigan   (2006-10-30)


recomb. backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 48
    Illegal XhoI site found at 66
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Polylinker restriction sites must be unique to vector plasmid (modified pBR322)


Source

Annealed oligonucleotides from Invitrogen

References